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| Pages: 41
ANTIFUNGAL ACTIVITY OF LOCALLY MADE SOAP ON DERMATOPHYTES ISOLATED FROM BARBING EQUIPMENT
ABSTRACT
The comparative study on the effect of the locally made black soap and Dudu osun soap on dermatophytes isolated from barbing equipment using agar well diffusion method. The fungi isolated from the barbing equipment are Trichophyton rubrum (56.25%), Microsporum canis(28.13%), and Trichophyton mentagrophytes (15.63%). The isolates were treated with Dudu Osun and Black soap, the result showed that Trichophyton rubrum was resistance to 100% concentration of dudu soap but has a high growth level for 200, 300, and 400 concentration. In its treatment with black soap it was sensitive to all concentration. Trichophyton mentagrophyteswas sensitive to 100% and 400% concentration but has a high growth at 200% and 300% concentration in the treatment of dudu soap. It showed sensitivity at 200 and 300 concentation and a high growth at 100 and 400 concentration for the treatment of black soap treatment.Microsporum canis was sensitive to dudu soap treatment at 100% and had a high growth at 200%, 300%, and 400% concentration. In its treatment with black soap. It showed sensitive results at 200% and 300% but a highly growth on 100% and 400%. All controls had maximum growth. The results obtained revealed that the traditional black soap exhibited a high level of antimicrobial activities resulting in greater zones of inhibition against dudu osun soap. Hence, African black soap through this study can thus, be recommended for use since it has potentials of treating hair and skin diseases.
CHAPTER ONE
1.1 INTRODUCTION
The term normal microbial flora or microbiota denotes the population of microorganism that inhibits the skin and mucous membranes of healthy normal persons. Research has shown that this normal flora now referred to as normal microbiota provide a first line of defense against microbial pathogens, assist in digestion, play a role in toxin-degradation, and contribute to maturation of the immune system. Shifts in the normal microbiota or stimulations of inflammation by these commensals may cause disease as inflammatory bowel disease (Tachibana, 1976). The density and composition of the normal flora of skin vary with anatomical location. The makeup of the normal flora depends, upon various factors including genetic, sex, age, stress, nutrition and diet of the individuals. Human skin is subject to degenerative changes due to daily exposure to environment and the impact of microorganisms. Several studies have been carried out to determine the normal flora of the skin. (Marhall et al., 1987)Presented a comprehensive review of the normal flora of the foot. He stated that the flora of the foot is similar to that found in the other skin site. (Marples, 1992 )Believe that some differences exist between foot flora and other skin site. Black soap has anti-aging properties and can reduce fine lines and wrinkles for youthful, smoothskin. Dark spots and blemishes are evened out and the natural ingredients effectively cleanse and deodorize. Black soap does not
ABSTRACT
The comparative study on the effect of the locally made black soap and Dudu osun soap on dermatophytes isolated from barbing equipment using agar well diffusion method. The fungi isolated from the barbing equipment are Trichophyton rubrum (56.25%), Microsporum canis(28.13%), and Trichophyton mentagrophytes (15.63%). The isolates were treated with Dudu Osun and Black soap, the result showed that Trichophyton rubrum was resistance to 100% concentration of dudu soap but has a high growth level for 200, 300, and 400 concentration. In its treatment with black soap it was sensitive to all concentration. Trichophyton mentagrophyteswas sensitive to 100% and 400% concentration but has a high growth at 200% and 300% concentration in the treatment of dudu soap. It showed sensitivity at 200 and 300 concentation and a high growth at 100 and 400 concentration for the treatment of black soap treatment.Microsporum canis was sensitive to dudu soap treatment at 100% and had a high growth at 200%, 300%, and 400% concentration. In its treatment with black soap. It showed sensitive results at 200% and 300% but a highly growth on 100% and 400%. All controls had maximum growth. The results obtained revealed that the traditional black soap exhibited a high level of antimicrobial activities resulting in greater zones of inhibition against dudu osun soap. Hence, African black soap through this study can thus, be recommended for use since it has potentials of treating hair and skin diseases.
CHAPTER ONE
1.1 INTRODUCTION
The term normal microbial flora or microbiota denotes the population of microorganism that inhibits the skin and mucous membranes of healthy normal persons. Research has shown that this normal flora now referred to as normal microbiota provide a first line of defense against microbial pathogens, assist in digestion, play a role in toxin-degradation, and contribute to maturation of the immune system. Shifts in the normal microbiota or stimulations of inflammation by these commensals may cause disease as inflammatory bowel disease (Tachibana, 1976). The density and composition of the normal flora of skin vary with anatomical location. The makeup of the normal flora depends, upon various factors including genetic, sex, age, stress, nutrition and diet of the individuals. Human skin is subject to degenerative changes due to daily exposure to environment and the impact of microorganisms. Several studies have been carried out to determine the normal flora of the skin. (Marhall et al., 1987)Presented a comprehensive review of the normal flora of the foot. He stated that the flora of the foot is similar to that found in the other skin site. (Marples, 1992 )Believe that some differences exist between foot flora and other skin site. Black soap has anti-aging properties and can reduce fine lines and wrinkles for youthful, smoothskin. Dark spots and blemishes are evened out and the natural ingredients effectively cleanse and deodorize. Black soap does not
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